However, these new technologies require new workflows and an understanding of their limitations and strengths. The chameleon instrument with its self-wicking grids and fast-plunge freezing represents a shift towards a robust, automated, and highly controllable future for specimen preparation.
#The division 2 chameleon manual#
The process has transformed structural biology, but it is largely manual and can require extensive optimisation for each protein sample. The specimen preparation process is a key determinant in the success of any cryo electron microscopy (cryoEM) structural study and until recently had remained largely unchanged from the initial designs of Jacques Dubochet and others in the 1980s.
8Diamond Light Source Ltd., Harwell Science Campus, Didcot, United Kingdom.7MIT.nano, Massachusetts Institute of Technology, Cambridge, MA, United States.6Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, United States.5Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, United States.3Division of Structural Biology, The Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.2Structural Biology, The Rosalind Franklin Institute, Harwell Science Campus, Didcot, United Kingdom.1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, United States.Levitz 1 †, Miriam Weckener 2,3 †, Ivan Fong 4, James H.